Composite

Part:BBa_K3196033:Design

Designed by: XinRan Rao   Group: iGEM19_HUST-China   (2019-10-16)


FLO10-αpro-Geneart Laccase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 349
    Illegal BglII site found at 886
    Illegal BglII site found at 1582
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is an improvement of part Baa_K500002, which codes the Geneart Laccase. Geneart Laccase is a polyphenol oxidase, which belongs to the family of blue multicopper oxidases. Laccases oxidize a broad range of substrates, preferably phenolic compounds. As we found in our project, the signal peptide has a strong influence on protein secretory. We have references show that the FLO10-αpro contributes a lot to the laccase secretory in yeast. As a part, we want to amplify the response of the promoter.


Source

BBa_K500002

References

Yiping Shi, Xue Zhou, Meirong Nu, Jianhui Lin, Yong Tao, Jianzhong Huang. Effect of signal peptides on the expression of laccase in Pichia pastoris. Acta Microbiologica Sinica, 54(12): 1446-1452.